Characterisation of cyclin D1 down-regulation in coronavirus infected cells.
Identifieur interne : 003756 ( Main/Exploration ); précédent : 003755; suivant : 003757Characterisation of cyclin D1 down-regulation in coronavirus infected cells.
Auteurs : Sally M. Harrison [Royaume-Uni] ; Brian K. Dove ; Lisa Rothwell ; Pete Kaiser ; Ian Tarpey ; Gavin Brooks ; Julian A. HiscoxSource :
- FEBS letters [ 0014-5793 ] ; 2007.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN messager, ARN viral, Cycline D1.
- génétique : Cycline D1.
- métabolisme : ARN messager, ARN viral, Cycline D1.
- Animaux, Cellules Vero, Coronavirus, RT-PCR, Régulation négative, Technique de Western, Virus de la bronchite infectieuse.
English descriptors
- KwdEn :
- Animals, Blotting, Western, Chlorocebus aethiops, Coronavirus, Cyclin D1 (analysis), Cyclin D1 (genetics), Cyclin D1 (metabolism), Down-Regulation, Infectious bronchitis virus, RNA, Messenger (analysis), RNA, Messenger (metabolism), RNA, Viral (analysis), RNA, Viral (metabolism), Reverse Transcriptase Polymerase Chain Reaction, Vero Cells.
- MESH :
- chemical , analysis : Cyclin D1, RNA, Messenger, RNA, Viral.
- chemical , genetics : Cyclin D1.
- chemical , metabolism : Cyclin D1, RNA, Messenger, RNA, Viral.
- Animals, Blotting, Western, Chlorocebus aethiops, Coronavirus, Down-Regulation, Infectious bronchitis virus, Reverse Transcriptase Polymerase Chain Reaction, Vero Cells.
Abstract
The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. Confocal microscopy revealed that cyclin D1 decreased in IBV-infected cells as infection progressed and inhibition studies indicated that a population of cyclin D1 could be targeted for degradation by a virus mediated pathway. In contrast to the SARS-coronavirus, IBV nucleocapsid protein did not interact with cyclin D1.
DOI: 10.1016/j.febslet.2007.02.039
PubMed: 17359980
Affiliations:
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Le document en format XML
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Harrison</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT</wicri:regionArea><orgName type="university">Université de Leeds</orgName><placeName><settlement type="city">Leeds</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Yorkshire-et-Humber</region></placeName></affiliation></author><author><name sortKey="Dove, Brian K" sort="Dove, Brian K" uniqKey="Dove B" first="Brian K" last="Dove">Brian K. 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Hiscox</name></author></analytic><series><title level="j">FEBS letters</title><idno type="ISSN">0014-5793</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Chlorocebus aethiops</term><term>Coronavirus</term><term>Cyclin D1 (analysis)</term><term>Cyclin D1 (genetics)</term><term>Cyclin D1 (metabolism)</term><term>Down-Regulation</term><term>Infectious bronchitis virus</term><term>RNA, Messenger (analysis)</term><term>RNA, Messenger (metabolism)</term><term>RNA, Viral (analysis)</term><term>RNA, Viral (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (analyse)</term><term>ARN messager (métabolisme)</term><term>ARN viral (analyse)</term><term>ARN viral (métabolisme)</term><term>Animaux</term><term>Cellules Vero</term><term>Coronavirus</term><term>Cycline D1 (analyse)</term><term>Cycline D1 (génétique)</term><term>Cycline D1 (métabolisme)</term><term>RT-PCR</term><term>Régulation négative</term><term>Technique de Western</term><term>Virus de la bronchite infectieuse</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Cyclin D1</term><term>RNA, Messenger</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cyclin D1</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cyclin D1</term><term>RNA, Messenger</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN messager</term><term>ARN viral</term><term>Cycline D1</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cycline D1</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>ARN viral</term><term>Cycline D1</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Chlorocebus aethiops</term><term>Coronavirus</term><term>Down-Regulation</term><term>Infectious bronchitis virus</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Coronavirus</term><term>RT-PCR</term><term>Régulation négative</term><term>Technique de Western</term><term>Virus de la bronchite infectieuse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. Confocal microscopy revealed that cyclin D1 decreased in IBV-infected cells as infection progressed and inhibition studies indicated that a population of cyclin D1 could be targeted for degradation by a virus mediated pathway. In contrast to the SARS-coronavirus, IBV nucleocapsid protein did not interact with cyclin D1.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Yorkshire-et-Humber</li></region><settlement><li>Leeds</li></settlement><orgName><li>Université de Leeds</li></orgName></list><tree><noCountry><name sortKey="Brooks, Gavin" sort="Brooks, Gavin" uniqKey="Brooks G" first="Gavin" last="Brooks">Gavin Brooks</name><name sortKey="Dove, Brian K" sort="Dove, Brian K" uniqKey="Dove B" first="Brian K" last="Dove">Brian K. Dove</name><name sortKey="Hiscox, Julian A" sort="Hiscox, Julian A" uniqKey="Hiscox J" first="Julian A" last="Hiscox">Julian A. Hiscox</name><name sortKey="Kaiser, Pete" sort="Kaiser, Pete" uniqKey="Kaiser P" first="Pete" last="Kaiser">Pete Kaiser</name><name sortKey="Rothwell, Lisa" sort="Rothwell, Lisa" uniqKey="Rothwell L" first="Lisa" last="Rothwell">Lisa Rothwell</name><name sortKey="Tarpey, Ian" sort="Tarpey, Ian" uniqKey="Tarpey I" first="Ian" last="Tarpey">Ian Tarpey</name></noCountry><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Harrison, Sally M" sort="Harrison, Sally M" uniqKey="Harrison S" first="Sally M" last="Harrison">Sally M. Harrison</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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